Experiment to determine the presence of a single nucleotide Lab Report

Experiment to determine the presence of a single nucleotide polymorphism in a gene - Lab Report Example Factors such as the recombination of genetics and the rate or mutation play a big role in determining the density of SNP (Kwak, J. 2007). The density of SNP could be predicted by the microsatellites presence. The microsatellites AT nucleotides are the potent SNP predictors of density with the repeated long tracts that can be found in areas of reduced SNP density and low content of GC nucleotides. SNP in a population is assigned the frequency of a minor allele (Shastry, B. 2002). This involves the less frequency of allele for SNP. In order to understand the occurrence of a single nucleotide polymorphism, an experiment was set to determine the presence of a single nucleotide polymorphism in a gene. The materials that were used in the experiment include: Sample 1, which was the Lambda DNA(ÃŽ »DNA) that was digested with Hindlll, Sample 2, which was a subjected DNA*PCR amplified for undigested WMIN gene, and sample 3, which was a subjected DNA * PCR amplified for the gene WMIN that was digested using Hindlll. The loading buffer was added to the provided samples. The contents of the loading buffer are a tracking dye, bromophenol blue, and glycerol that made possible for the samples to sink into the well of loading on the gel. During the experiment, the disposable gloves were worn to reduce the contamination of DNAase from the fingers. About 50 mL 0.8% w/v agarose gel was prepared. Gelred was added to agarose gel (Su MC, Y. 2008). The agarose gel was placed into the apparatus of electrophoresis, having the wells on the black end of the cathode. TBE running buffer was added to cover the gel to a level of 1-2 mm over the surface. About 4 µl quantity of samples having 100ng DNA was loaded from sample one to three inside the wells using P20 Gilson. The lid was put on, the power turned on and adjusted to about 100 v. The power was run for approximately 30 minutes up to when the blue tracker dye was about  ¾ of the gel. The power supply was turned off and the

The problems related to the killing by the Einsatzgruppen Term Paper

The problems related to the killing by the Einsatzgruppen - Term Paper Example An example was police battalion 101 that was charged with extermination of the polish Jewry, and ‘pacifying’ their regions (Goldhagen, 1996, 239). Einsatzgruppen original role is that they often accompanied the military, with instruction to combat anyone behind the frontline, hostile, or bent to sabotage the Reich. However, what they actually did is that they were involved in the deportation, persecution and torture of the Jews in the most despicable manner .In addition, they were often employed in retributive killings whenever Germany lost a soldier in the occupied territory (Goldhagen, 1996, 240). According to Goldhagen (1996, 247) the method employed was shooting their victims in huge trenches, abandoned quarries, ravines and ditches. However, problems developed with this mode of execution. Not all members of these squads were comfortable on the cruelty meted out on their victims. Cold blood executions, especially of women and children were taking a toll on the mental state of the executioners. Some committed suicide, some went mad, and others resulted to massive drinking to combat the memories (Hoss, 1996, 197). For instance, according to Goldhagen (1996, 240), commander of Battalion 101, major Trapp was shaken and wept, following the retributive killing of 200 poles. ... He suffered emotionally and mentally but could not show emotion openly. He became dissatisfied with himself and his main responsibility. This negates the theory advanced by Goldhagen (1996) that all Germans willfully participated in the killing to quench their cruelty and their hate for the Jews. Goldhagen (1996, 246) argues that the Germans wanted to publicly humiliate the Jews, by example shaving their beards which was a symbol of their manhood. Therefore, they deemed their role as that of obeying orders given by Hitler without questioning the morality of the orders. According to Hoss (1996, 161), many people secretly doubted Hitler’s decisions but he could not reveal this. This tally with Browning’s (1995, 99) account, who depicts one Major Trapp as crying the whole day, after he gave an order for the mass killing of innocent Jews. However, according to Goldhagen (1996, 249), all those who participated in the killings could walk away or withdraw from the assignment w ithout any ramifications. In fact, he gives account of an officer who was promoted, even after he had withdrawn from killing assignments. This is contradicted by Browning (1995) adds that those who participated in the killings did not know the specifics of their assignments until it was very late. In fact, Hoss (1996, 163) narrates that after held discussions with Eichmann, realized that there was no escaping the conflict at all. However, according to Borowski (1995, 24) those who participated in the killing viewed themselves to be killing under duress. They were forced to do so. For instance, he narrates a case where an SS officer threatened to shoot him for failure to carry a dead child. In addition, Browning (1996, 29) argues that participation was out of fear of being labeled